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KMID : 1007520110200061747
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Food Science and Biotechnology
2011 Volume.20 No. 6 p.1747 ~ p.1750
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Comparison of ELISA and HPLC Methods for the Determination of Biogenic Amines in Commercial Doenjang and Gochujang
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Kim Tae-Kyun
Lee Jae-Ick
Kim Jin-Hyo
Mah Jae-Hyung
Hwang Han-Joon
Kim Young-Wan
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Abstract
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In order to conduct fast detection of biogenic amines (BAs) in doenjang and gochujang, a competitive direct enzyme-linked immunosorbent assay (CD-ELISA) for histamine was compared with HPLC analysis. The histamine contents were determined from 0.7 to 420 mg/kg by CD-ELISA and 2.2 to 382.4 mg/kg by HPLC. Both methods showed a good agreement for histamine analysis (R=0.969). In addition, the histamine contents by CDELISA were correlated to the sum of 7 major BAs in the samples (R=0.910), suggesting that the CD-ELISA can be used as a rapid indicator for total BAs in doenjang and gochujang.
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KEYWORD
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biogenic amine, doenjang, gochujang, competitive direct enzyme-linked immunosorbent assay, HPLC
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